Methods & Protocols
If you would like to share your robust protocols with your colleagues, please email them to enquiry@stemcell.edu.sg Isolation of Tissue Stem Cells
- SP cell sorting with Hoechst dye (M.A. Goodell laboratory)
(Download) - Isolation of stem cells from muscle (M.A. Goodell laboratory)
(Download)
Gene Delivery into Stem Cells
- siRNA electroporation of mouse bone marrow progenitors (M.A. Goodell laboratory)
(Download) - Electroporation of Murine Embryonic Stem Cells: A Step-by-Step Guide (Dennie M. Tompers, Patricia A. Labosky Stem Cells 22: 243)
(Download)
Human Embryonic Stem Cells
- Methods for Expansion of Human Embryonic Stem Cells (Sun Kyung Oh et al., Stem Cells 23: 605)
(Download) - Derivation and culture of hESC (Harvard lines, Cowan et al., N Engl J Med. 350:1353)
(Download) - Complete methodology manual from ES Cell International.
(Download)
Additional protocols can be found on the NIH (http://stemcells.nih.gov/research/protocols.asp) and ISSCR web sites (http://www.isscr.org/science/sclines.htm, bottom of the page)
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